Protocol Protocol for Primary Mouse Hepatocyte Isolation

Primary hepatocytes are a vital tool in various biomedical research disciplines, serving as an ex vivo model for liver physiology. Obtaining high yields of viable primary mouse hepatocytes is technically challenging, limiting their use. Here, we present an improved protocol based on the classic two-step collagenase perfusion technique. The liver is washed by perfusion, hepatocytes are dissoci-ated by collagenase, separated from other cells, and cultured. This protocol was optimized to significantly reduce procedure duration and improve hepato-cyte yield and viability.