Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions

被引：0
|
作者：
Sergey Rodin
Liselotte Antonsson
Outi Hovatta
Karl Tryggvason
机构：
[1] Karolinska Institute,Department of Medical Biochemistry and Biophysics, Division of Matrix Biology
[2] Intervention and Technology,Department of Clinical Sciences, Division of Obstetrics and Gynecology
[3] Karolinska Institute and Karolinska University Hospital,undefined
[4] Cardiovascular and Metabolic Disorders Program,undefined
[5] Duke-NUS (National University of Singapore),undefined
来源：
Nature Protocols | 2014年 / 9卷
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D O I：
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学科分类号：
摘要：
Coating of cell culture plates with laminin-521 allows culturing of human pluripotent stem cells and, in combination with E-cadherin, their cloning in individual wells.
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收藏
页码：2354 / 2368
页数：14
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