Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions

被引：0

作者：

Sergey Rodin

Liselotte Antonsson

Outi Hovatta

Karl Tryggvason

机构：

[1] Karolinska Institute,Department of Medical Biochemistry and Biophysics, Division of Matrix Biology

[2] Intervention and Technology,Department of Clinical Sciences, Division of Obstetrics and Gynecology

[3] Karolinska Institute and Karolinska University Hospital,undefined

[4] Cardiovascular and Metabolic Disorders Program,undefined

[5] Duke-NUS (National University of Singapore),undefined

来源：

Nature Protocols | 2014年 / 9卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Coating of cell culture plates with laminin-521 allows culturing of human pluripotent stem cells and, in combination with E-cadherin, their cloning in individual wells.

引用

页码：2354 / 2368

页数：14

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