Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension

被引：0

作者：

Paul Lebel

Aakash Basu

Florian C Oberstrass

Elsa M Tretter

Zev Bryant

机构：

[1] Stanford University,Department of Applied Physics

[2] Stanford University,Department of Bioengineering

[3] University of California,Department of Molecular and Cell Biology

[4] Stanford University Medical Center,Department of Structural Biology

[5] Present addresses: Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience,undefined

[6] The Rockefeller University,undefined

[7] New York,undefined

[8] New York,undefined

[9] USA (A.B.),undefined

[10] and Nurix Inc.,undefined

[11] San Francisco,undefined

[12] California,undefined

[13] USA (E.M.T.).,undefined

来源：

Nature Methods | 2014年 / 11卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Single-molecule structural transitions involving DNA twisting can be measured with substantially greater spatiotemporal resolution than previously possible with a gold rotor bead tracking (AuRBT) method. This approach uses magnetic tweezers and evanescent darkfield microscopy to track a gold nanoparticle probe attached to a DNA molecule.

引用

页码：456 / 462

页数：6

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