Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension

被引：0
|
作者：
Paul Lebel
Aakash Basu
Florian C Oberstrass
Elsa M Tretter
Zev Bryant
机构：
[1] Stanford University,Department of Applied Physics
[2] Stanford University,Department of Bioengineering
[3] University of California,Department of Molecular and Cell Biology
[4] Stanford University Medical Center,Department of Structural Biology
[5] Present addresses: Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience,undefined
[6] The Rockefeller University,undefined
[7] New York,undefined
[8] New York,undefined
[9] USA (A.B.),undefined
[10] and Nurix Inc.,undefined
[11] San Francisco,undefined
[12] California,undefined
[13] USA (E.M.T.).,undefined
来源：
Nature Methods | 2014年 / 11卷
关键词：
D O I：
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中图分类号：
学科分类号：
摘要：
Single-molecule structural transitions involving DNA twisting can be measured with substantially greater spatiotemporal resolution than previously possible with a gold rotor bead tracking (AuRBT) method. This approach uses magnetic tweezers and evanescent darkfield microscopy to track a gold nanoparticle probe attached to a DNA molecule.
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页码：456 / 462
页数：6
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