Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics

被引：0

作者：

Schneider J. ^{[1
,2
]}

Zahn J. ^{[1
,2
]}

Maglione M. ^{[3
]}

Sigrist S.J. ^{[3
]}

Marquard J. ^{[1
,2
]}

Chojnacki J. ^{[4
]}

Kräusslich H.-G. ^{[4
]}

Sahl S.J. ^{[5
]}

Engelhardt J. ^{[1
,2
]}

Hell S.W. ^{[1
,2
,5
]}

机构：

[1] German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg

[2] Bioquant Center, Heidelberg

[3] Institute for Biology and Genetics, Free University of Berlin, Berlin

[4] Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg

[5] Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen

来源：

Nature Methods | 2015年 / 12卷 / 9期

关键词：

D O I：

10.1038/nmeth.3481

中图分类号：

学科分类号：

摘要：

Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution. © 2015 Nature America, Inc.

引用

下载

页码：827 / 830

页数：3

共 50 条

[31] Suppressing background noise in STED optical nanoscopy
Jeong, Sejoo
Kim, Jaeyong
Lee, Jong-Chan
JOURNAL OF THE KOREAN PHYSICAL SOCIETY, 2021, 78 (05) : 401 - 407
[32] Closing in on video-rate STED nanoscopy
Nature Methods, 2015, 12 (3) : 173 - 173
[33] STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA
Heller I.
Sitters G.
Broekmans O.D.
Farge G.
Menges C.
Wende W.
Hell S.W.
Peterman E.J.G.
Wuite G.J.L.
Nature Methods, 2013, 10 (9) : 910 - 916
[34] Visualizing ultrafast photothermal dynamics with decoupled optical force nanoscopy
Wang, Hanwei
Meyer, Sean M.
Murphy, Catherine J.
Chen, Yun-Sheng
Zhao, Yang
NATURE COMMUNICATIONS, 2023, 14 (01)
[35] Visualizing ultrafast photothermal dynamics with decoupled optical force nanoscopy
Hanwei Wang
Sean M. Meyer
Catherine J. Murphy
Yun-Sheng Chen
Yang Zhao
Nature Communications, 14
[36] Ultrafast coherent nanoscopy
Chen, Xue-Wen
Mohammadi, Ahmad
Ghasemi, Amir Hossein Baradaran
Agio, Mario
MOLECULAR PHYSICS, 2013, 111 (20) : 3003 - 3012
[37] STED Nanoscopy Combined with Optical Tweezers Reveals Spatial Dynamics of Proteins on Densely Covered DNA
Sitters, Gerrit
Heller, Iddo
Broekmans, Onno D.
Farge, Geraldine A.
Menges, Carolin
Wende, Wolfgang
Hell, Stefan W.
Peterman, Erwin J. G.
Wuite, Gijs J. L.
BIOPHYSICAL JOURNAL, 2013, 104 (02) : 210A - 211A
[38] Live-cell STED nanoscopy of mitochondrial cristae
Till Stephan
Axel Roesch
Dietmar Riedel
Stefan Jakobs
Scientific Reports, 9
[39] Accurate Background Subtraction in STED Nanoscopy by Polarization Switching
Lee, Jong-Chan
Ma, Ye
Han, Kyu Young
Ha, Taekjip
ACS PHOTONICS, 2019, 6 (07) : 1789 - 1797
[40] Quantifying Molecule Numbers in STED/RESOLFT Fluorescence Nanoscopy
Keller-Findeisen, Jan
Sahl, Steffen J.
Hell, Stefan W.
NANOSCALE PHOTONIC IMAGING, 2020, 134 : 205 - 226

← 1 2 3 4 5 →