Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics

被引：0
|
作者：
Schneider J. [1 ,2 ]
Zahn J. [1 ,2 ]
Maglione M. [3 ]
Sigrist S.J. [3 ]
Marquard J. [1 ,2 ]
Chojnacki J. [4 ]
Kräusslich H.-G. [4 ]
Sahl S.J. [5 ]
Engelhardt J. [1 ,2 ]
Hell S.W. [1 ,2 ,5 ]
机构：
[1] German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg
[2] Bioquant Center, Heidelberg
[3] Institute for Biology and Genetics, Free University of Berlin, Berlin
[4] Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg
[5] Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen
来源：
Nature Methods | 2015年 / 12卷 / 9期
关键词：
D O I：
10.1038/nmeth.3481
中图分类号：
学科分类号：
摘要：
Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution. © 2015 Nature America, Inc.
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页码：827 / 830
页数：3
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