Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

被引：0

作者：

Beate Neumann

Thomas Walter

Jean-Karim Hériché

Jutta Bulkescher

Holger Erfle

Christian Conrad

Phill Rogers

Ina Poser

Michael Held

Urban Liebel

Cihan Cetin

Frank Sieckmann

Gregoire Pau

Rolf Kabbe

Annelie Wünsche

Venkata Satagopam

Michael H. A. Schmitz

Catherine Chapuis

Daniel W. Gerlich

Reinhard Schneider

Roland Eils

Wolfgang Huber

Jan-Michael Peters

Anthony A. Hyman

Richard Durbin

Rainer Pepperkok

Jan Ellenberg

机构：

[1] MitoCheck Project Group,Division of Theoretical Bioinformatics

[2] ,undefined

[3] Gene Expression and,undefined

[4] ,undefined

[5] Cell Biology/Biophysics Units,undefined

[6] Structural and,undefined

[7] ,undefined

[8] Computational Biology Unit,undefined

[9] European Molecular Biology Laboratory (EMBL),undefined

[10] Meyerhofstrasse 1,undefined

[11] D-69117 Heidelberg,undefined

[12] Germany ,undefined

[13] Wellcome Trust Sanger Institute,undefined

[14] Wellcome Trust Genome Campus,undefined

[15] Hinxton,undefined

[16] Cambridge CB10 1HH,undefined

[17] UK ,undefined

[18] Max Planck Institute for Molecular Cell Biology and Genetics,undefined

[19] Pfotenhauerstrasse 108,undefined

[20] D-01307 Dresden,undefined

[21] Germany ,undefined

[22] Institute of Biochemistry,undefined

[23] Swiss Federal Institute of Technology Zurich (ETHZ),undefined

[24] Schafmattstrasse 18,undefined

[25] CH-8093 Zurich,undefined

[26] Switzerland ,undefined

[27] Leica Microsystems CMS GmbH,undefined

[28] Am Friedensplatz 3,undefined

[29] D-68165 Mannheim,undefined

[30] Germany ,undefined

[31] European Bioinformatics Institute,undefined

[32] European Molecular Biology Laboratory,undefined

[33] German Cancer Research Center,undefined

[34] Im Neuenheimer Feld 267,undefined

[35] D-69120 Heidelberg,undefined

[36] Germany,undefined

[37] Institute for Molecular Pathology,undefined

[38] Dr Bohr Gasse 7,undefined

[39] A-1030 Vienna,undefined

[40] Austria ,undefined

[41] Present addresses: MitoCheck Project Group,undefined

[42] European Molecular Biology Laboratory (EMBL),undefined

[43] Meyerhofstrasse 1,undefined

[44] D-69117 Heidelberg,undefined

[45] Germany (J.-K.H.); BIOQUANT Centre University Heidelberg,undefined

[46] INF 267,undefined

[47] D-69120 Heidelberg,undefined

[48] Germany (H.E.); 3-V Biosciences GmbH,undefined

[49] Wagistrasse 27,undefined

[50] 8952 Schlieren,undefined

来源：

Nature | 2010年 / 464卷

关键词：

D O I：

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中图分类号：

学科分类号：

摘要：

Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.

引用

页码：721 / 727

页数：6

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