Photothermal bleaching in time-lapse photoacoustic microscopy

被引：13
|
作者：
Gao, Liang [1 ]
Wang, Lidai [1 ]
Li, Chiye [1 ]
Garcia-Uribe, Alejandro [1 ]
Wang, Lihong V. [1 ]
机构：
[1] Washington Univ, Dept Biomed Engn, St Louis, MO 63130 USA
来源：
JOURNAL OF BIOPHOTONICS | 2013年 / 6卷 / 6-7期
基金：
美国国家卫生研究院;
关键词：
photoacoustic microscopy; nanoparticle; photothermal bleaching; timelapse imaging; FLUORESCENCE MICROSCOPY; GOLD NANOPARTICLES; LIVING CELLS; TOMOGRAPHY; DYNAMICS; SURFACE;
D O I：
10.1002/jbio.201200184
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We studied the phenomenon of photothermal bleaching - a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy, pulse duration, and the absorber's size was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
引用
收藏
页码：543 / 548
页数：6
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