Quantification of Saccharomyces cerevisiae viability using BacLight

被引：0
|
作者：
Tong Zhang
Herbert H.P. Fang
机构：
[1] University of Hong Kong,Environmental Biotechnology Laboratory, Department of Civil Engineering The
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
Light; cell; viability; yeast;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
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页码：989 / 992
页数：3
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