Evaluation of cell numbers and viability of Saccharomyces cerevisiae by different counting methods

被引：20
|
作者：
Fiala, J
Lloyd, DR
Rychtera, M
Kent, CA [1 ]
Al-Rubeai, M
机构：
[1] Univ Birmingham, Sch Chem Engn, Ctr Bioproc Engn, Birmingham B15 2TT, W Midlands, England
[2] Inst Chem Technol, Dept Fermentat Chem & Bioengn, Prague 16628 6, Czech Republic
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 11期
基金：
英国生物技术与生命科学研究理事会;
关键词：
cell number; Coulter principle; flow cytometry; viability; yeast;
D O I：
10.1023/A:1008974419342
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Four counting methods (two flow cytometry, one Coulter principle, one microscopic) are compared for measuring cell density and viability of batch-grown yeast. All gave adequate precision in measuring total cell density with no systematic difference between methods. However, the promise of flow cytometry as a rapid means of determining both quantity and quality of a cell population is shown.
引用
收藏
页码：787 / 795
页数：9
相关论文
共 50 条
  • [1] Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae
    Zhuk A.S.
    Stepchenkova E.I.
    Pavlov Y.I.
    Inge-Vechtomov S.G.
    Cell and Tissue Biology, 2017, 11 (2) : 111 - 122
  • [2] INFLUENCE OF OXYGEN RADICALS ON THE CELL VIABILITY OF SACCHAROMYCES CEREVISIAE WITH DIFFERENT LEVEL OF THE METALLOTHIONEIN GENE-EXPRESSION
    SHARONOV, BP
    KISELEV, OI
    KONDRATIEVA, LD
    PASECHNIK, VA
    DOKLADY AKADEMII NAUK SSSR, 1989, 307 (06): : 1490 - 1493
  • [3] Function of trehalose and glycogen in cell cycle progression and cell viability in Saccharomyces cerevisiae
    Silljé, HHW
    Paalman, JWG
    ter Schure, EG
    Olsthoorn, SQB
    Verkleij, AJ
    Boonstra, J
    Verrips, CT
    JOURNAL OF BACTERIOLOGY, 1999, 181 (02) : 396 - 400
  • [4] Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae
    Sasaki, K
    Abid, MR
    Miyazaki, M
    FEBS LETTERS, 1996, 384 (02) : 151 - 154
  • [5] Effect of copper imbalance in cell viability of the budding yeast Saccharomyces cerevisiae
    Strubbe, Jasiel
    Velez-Burgos, Yaritza
    Quinones, Jose Rodriguez
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 251
  • [6] Deoxyhypusine synthase activity is essential for cell viability in the yeast Saccharomyces cerevisiae
    Park, MH
    Joe, YA
    Kang, KR
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (03) : 1677 - 1683
  • [7] Ergosterol determination in Saccharomyces cerevisiae. Comparison of different methods
    Lamacka, M
    Sajbidor, J
    BIOTECHNOLOGY TECHNIQUES, 1997, 11 (10) : 723 - 725
  • [8] CONSTANT COPY NUMBERS OF PLASMIDS IN SACCHAROMYCES-CEREVISIAE HOSTS WITH DIFFERENT PLOIDIES
    TAKAGI, A
    CHUA, EN
    BOONCHIRD, C
    HARASHIMA, S
    OSHIMA, Y
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 23 (02) : 123 - 129
  • [9] Evaluation and Optimization of Metabolome Sample Preparation Methods for Saccharomyces cerevisiae
    Kim, Sooah
    Lee, Do Yup
    Wohlgemuth, Gert
    Park, Hyong Seok
    Fiehn, Oliver
    Kim, Kyoung Heon
    ANALYTICAL CHEMISTRY, 2013, 85 (04) : 2169 - 2176
  • [10] Quantification of Saccharomyces cerevisiae viability using BacLight
    Tong Zhang
    Herbert H.P. Fang
    Biotechnology Letters, 2004, 26 : 989 - 992
12345