Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels

被引：10
|
作者：
Izzo V. [1 ]
Costa M.A. [1 ]
Di Fiore R. [1 ]
Duro G. [1 ]
Bellavia D. [2 ]
Cascone E. [2 ]
Colombo P. [1 ]
Gioviale M.C. [3 ]
Barbieri R. [2 ]
机构：
[1] Istituto di Biomedicina e Immunologia Molecolare, C.N.R., 90153 Palermo
[2] Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, V.le delle Scienze
[3] Dipartimento GEN.UR.TO, Università di Palermo, Palermo
来源：
Immunity & Ageing | / 3卷 / 1期
关键词：
Glass Plate; Milled Surface; 16HBE Cell; Ammonium Persulfate; Polyacrylamide Solution;
D O I：
10.1186/1742-4933-3-7
中图分类号：
学科分类号：
摘要：
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms. © 2006 Izzo et al; licensee BioMed Central Ltd.
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