In-house and interlaboratory validation of a method for the extraction of DNA from pollen in honey

被引：0
|
作者：
Hans-Ulrich Waiblinger
Marc Ohmenhaeuser
Stefanie Meissner
Miriam Schillinger
Klaus Pietsch
Ottmar Goerlich
Joachim Mankertz
Kathrin Lieske
Hermann Broll
机构：
[1] Chemisches und Veterinäruntersuchungsamt (CVUA) Freiburg,
[2] Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL),undefined
[3] Bundesamt für Verbraucherschutz und Lebensmittelsicherheit,undefined
[4] Bundesinstitut für Risikobewertung,undefined
[5] Abteilung Lebensmittelsicherheit,undefined
来源：
Journal für Verbraucherschutz und Lebensmittelsicherheit | 2012年 / 7卷
关键词：
Honey; DNA extraction; Pollen; Validation; Practical LOD; genetically modified organism (GMO);
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this work, the in-house and interlaboratory validation of a DNA extraction method from pollen in an unifloral rape honey as well as several multifloral honeys is described. The amplifiability of plant and rape DNA amplifiable by real-time PCR was used as a parameter for the evaluation of the method. The practical (i.e., relative) limit of detection was used as a tool for assessing the suitability of the extraction method for further GMO analysis. In a collaborative study with 14 participating labs the results of the in-house validation could be confirmed. The amount of amplifiable plant and rape DNA varied depending on the type of honey. For rape honey, a mean practical LOD of 0.12 % was obtained.
引用
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页码：243 / 254
页数：11
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