Ring trial validation of a method for the extraction of DNA from soy lecithins

被引：6
|
作者：
Waiblinger, H. -U. [1 ]
Ernst, B. [1 ]
Graf, N. [2 ]
Pietsch, K. [1 ]
机构：
[1] Chem Veterinaruntersuchungsamt Freiburg, D-79114 Freiburg, Germany
[2] Inst Lebensmittelchem, Landesuntersuchgsamt Rheinland Pfalz, Trier, Germany
来源：
JOURNAL FUR VERBRAUCHERSCHUTZ UND LEBENSMITTELSICHERHEIT-JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY | 2007年 / 2卷 / 02期
关键词：
DNA extraction; soy lecithin; validation; practical LOD; genetically modified organism (GMO);
D O I：
10.1007/s00003-007-0166-y
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In this work an approach for ring trial validation of a DNA extraction method for further GMO analysis is described. Practical LOD was used as a keytool for assessing the suitability of the extraction method. in ring trial with soy lecithins, satisfactory results with practical LODs below 0.9% were achieved in 4 out of 5 samples. In.-house tests showed that this protocol - in a slightly extended version - can also be used to extract DNA from native rapeseed oils.
引用
收藏
页码：113 / 115
页数：3
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