Direct electrochemistry and electrocatalysis of hemoglobin immobilized by methacrylic acid

被引：0
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作者：
Junhui Xu
Jia Zhao
Fang Wang
Qing Lu
Yanxia Xu
Shengshui Hu
机构：
[1] Wuhan University,College of Chemistry and Molecular Sciences
[2] Chinese Academy of Sciences,State Key Laboratory of Transducer Technology
来源：
Russian Journal of Electrochemistry | 2008年 / 44卷
关键词：
hemoglobin; methacrylic acid; direct electrochemistry; electrocatalysis; biosensor;
D O I：
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中图分类号：
学科分类号：
摘要：
The direct electrochemistry of hemoglobin (Hb) incorporated in methacrylic acid (MAA) film on a paraffin-impregnated graphite electrode (PIGE) was described. A pair of well-defined and quasi-reversible cyclic voltametric peaks are obtained. The formal potentials (E0′) linearly depend on the pH of solution, indicating that the electron transfer was proton-coupled. Ultraviolet-visible (UV-Vis) spectra showed that the secondary structure of Hb in the MAA film was similar to individual Hb. The immobilized Hb retained its biological activity well and exhibited a nice response to the reduction of both NO2−, and H2O2, on the basis of which a new biosensor has been developed.
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