SENSE: a fast RNA-seq library preparation protocol with superior strand specificity

被引：0
|
作者：
Courtney Nadeau
Alexander Seitz
机构：
[1] Lexogen GmbH,
来源：
Nature Methods | 2012年 / 9卷 / 12期
关键词：
D O I：
10.1038/nmeth.f.361
中图分类号：
学科分类号：
摘要：
The SENSE mRNA-seq library preparation kit generates ready-to-sequence libraries from low amounts of total RNA within 4 h. SENSE libraries are significantly more strand specific (>99.9%) than leading alternatives and are virtually free of rRNA contamination. The protocol does not rely on RNA or cDNA fragmentation or additional kits for rRNA depletion, library amplification or size selection.
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