A rapid, directional RNA-seq library preparation workflow for Illumina® sequencing

被引：0
|
作者：
Jim Pease
Roy Sooknanan
机构：
[1] Epicentre (an Illumina company),
来源：
Nature Methods | 2012年 / 9卷
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摘要：
Most current RNA-seq library preparation methods are time-consuming, multistep processes. We describe a workflow that includes the Ribo-Zero™ and ScriptSeq™ v2 Kits that enables researchers to go from total RNA to cluster-ready RNA-seq libraries in less than 1 d. The RNA-seq libraries produced are virtually free of contaminating ribosomal RNA (rRNA) and provide for directional paired-end and multiplex sequencing on Illumina® sequencing platforms.
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