Upstream sequencing and functional characterization of the human cholinergic gene locus

被引：0
|
作者：
Sung Ho Hahm
Liangbiao Chen
Chirag Patel
Jeffrey Erickson
Tom I. Bonner
Eberhard Weihe
Martin K. H. Schäfer
Lee E. Eiden
机构：
[1] NIH,Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation
[2] NIH,Section on Genetics, NIMH
[3] Philipps University,Department of Anatomy and Cell Biology
[4] Louisiana State University,Center for Neuroscience
来源：
Journal of Molecular Neuroscience | 1997年 / 9卷
关键词：
Vesicular acetylcholine transporter; VAChT; cholinergic gene locus; choline acetyltransferase; ChAT; neuron-specific gene transcription;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The 5′ flanking region of the human VAChT gene was sequenced to approx 5350 bases upstream of the initiating methionine codon of the VAChT open reading frame (orf). The 5′ flanks of the human and rat cholinergic gene loci were compared to identify regions of local sequence conservation, and therefore of potential regulatory importance. Several discrete domains of high homology, including a cluster of far-upstreamcis-active consensus motifs, a neuronally restrictive silencer element consensus sequence, and additional conserved sequences within the putative nerve growth factor response domain of the locus, were identified.
引用
收藏
页码：223 / 236
页数：13
相关论文
共 50 条
  • [1] Upstream sequencing and functional characterization of the human cholinergic gene locus
    Hahm, SH
    Chen, LB
    Patel, C
    Erickson, J
    Bonner, TI
    Weihe, E
    Schafer, MKH
    Eiden, LE
    JOURNAL OF MOLECULAR NEUROSCIENCE, 1997, 9 (03) : 223 - 236
  • [2] The cholinergic gene locus
    Eiden, LE
    JOURNAL OF NEUROCHEMISTRY, 1998, 70 (06) : 2227 - 2240
  • [3] From the cholinergic gene locus to the cholinergic neuron
    Weihe, E
    Schafer, MKH
    Schutz, B
    Anlauf, M
    Depboylu, C
    Brett, C
    Chen, LB
    Eiden, LE
    JOURNAL OF PHYSIOLOGY-PARIS, 1998, 92 (5-6) : 385 - 388
  • [4] Regulation of the cholinergic gene locus
    Tanaka, H
    Shimojo, M
    Wu, DH
    Hersh, LB
    JOURNAL OF PHYSIOLOGY-PARIS, 1998, 92 (02) : 149 - 152
  • [5] Regulation of the cholinergic gene locus
    Berse, B
    Blusztajn, JK
    JOURNAL OF NEUROCHEMISTRY, 1997, 69 : S111 - S111
  • [6] The cholinergic gene locus in amphioxus: Molecular characterization and developmental expression patterns
    Candiani, Simona
    Lacalli, Thurston C.
    Parodi, Manuela
    Oliveri, Diana
    Pestarino, Mario
    DEVELOPMENTAL DYNAMICS, 2008, 237 (05) : 1399 - 1411
  • [7] ORGANIZATION OF THE HUMAN N-RAS LOCUS - CHARACTERIZATION OF A GENE LOCATED IMMEDIATELY UPSTREAM OF N-RAS
    NICOLAIEW, N
    TRIQUENEAUX, G
    DAUTRY, F
    ONCOGENE, 1991, 6 (05) : 721 - 730
  • [8] Identification and functional validation of a 5′ upstream regulatory sequence in the human tyrosinase gene homologous to the locus control region of the mouse tyrosinase gene
    Regales, L
    Giraldo, P
    García-DIaz, A
    Lavado, A
    Montoliu, L
    PIGMENT CELL RESEARCH, 2003, 16 (06): : 685 - 692
  • [9] CHARACTERIZATION OF THE HUMAN LYSYL OXIDASE GENE LOCUS
    SVINARICH, DM
    TWOMEY, TA
    MACAULEY, SP
    KREBS, CJ
    YANG, TP
    KRAWETZ, SA
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (20) : 14382 - 14387
  • [10] FUNCTIONAL IDENTIFICATION OF A VESICULAR ACETYLCHOLINE TRANSPORTER AND ITS EXPRESSION FROM A CHOLINERGIC GENE LOCUS
    ERICKSON, JD
    VAROQUI, H
    SCHAFER, MKH
    MODI, W
    DIEBLER, MF
    WEIHE, E
    RAND, J
    EIDEN, LE
    BONNER, TI
    USDIN, TB
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (35) : 21929 - 21932
12345