Upstream sequencing and functional characterization of the human cholinergic gene locus

被引：0

作者：

Sung Ho Hahm

Liangbiao Chen

Chirag Patel

Jeffrey Erickson

Tom I. Bonner

Eberhard Weihe

Martin K. H. Schäfer

Lee E. Eiden

机构：

[1] NIH,Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation

[2] NIH,Section on Genetics, NIMH

[3] Philipps University,Department of Anatomy and Cell Biology

[4] Louisiana State University,Center for Neuroscience

来源：

Journal of Molecular Neuroscience | 1997年 / 9卷

关键词：

Vesicular acetylcholine transporter; VAChT; cholinergic gene locus; choline acetyltransferase; ChAT; neuron-specific gene transcription;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The 5′ flanking region of the human VAChT gene was sequenced to approx 5350 bases upstream of the initiating methionine codon of the VAChT open reading frame (orf). The 5′ flanks of the human and rat cholinergic gene loci were compared to identify regions of local sequence conservation, and therefore of potential regulatory importance. Several discrete domains of high homology, including a cluster of far-upstreamcis-active consensus motifs, a neuronally restrictive silencer element consensus sequence, and additional conserved sequences within the putative nerve growth factor response domain of the locus, were identified.

引用

页码：223 / 236

页数：13