The selective visualization of lignin peroxidase, manganese peroxidase and laccase, produced by white rot fungi on solid media

被引：1
|
作者：
Won Youl Ryu
Moon Yup Jang
Moo Hwan Cho
机构：
[1] Yeungnam University,School of Chemical Engineering & Technology
来源：
Biotechnology and Bioprocess Engineering | 2003年 / 8卷 / 2期
关键词：
lignin peroxidase; manganese peroxidase; laccase; color additives; white rot fungi;
D O I：
10.1007/BF02940268
中图分类号：
学科分类号：
摘要：
A visual method for the selective screening of lignin degrading enzymes, produced by white rot fungi (WRF), was investigated by the addition of coloring additives to solid media. Of the additives used in the enzyme production media, guaiacol and RBBR could be used for the detection of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase. Syringaldazine and Acid Red 264 were able for the detection of both the MnP and laccase, and the LiP and laccase, respectively, and a combination of these two additives was able to detect each of the ligninases produced by the WRF on solid media.
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页码：130 / 134
页数：4
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