The selective visualization of lignin peroxidase, manganese peroxidase and laccase, produced by white rot fungi on solid media

被引：0
|
作者：
Ryu, WY [1 ]
Jang, MY [1 ]
Cho, MH [1 ]
机构：
[1] Yeungnam Univ, Sch Chem Engn & Technol, Gyongbuk 712749, South Korea
来源：
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2003年 / 8卷 / 02期
关键词：
lignin peroxidase; manganese peroxidase; laccase; color additives; white rot fungi;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A visual method for the selective screen in g of lignin degrading enzymes, produced by white rot fungi (WRF), was investigated by the addition of coloring additives to solid media. Of the additives used in the enzyme production media, guaiacol and RBBR could be used for the detection of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase. Syringaldazine and Acid Red 264 were able for the detection of both the MnP and laccase, and the UP and laccase, respectively, and a combination of these two additives was able to detect each of the ligninases produced by the WRF on solid media.
引用
收藏
页码：130 / 134
页数：5
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