Isolation and characterization of twenty-one polymorphic microsatellite loci for Polycarpa aurata using third generation sequencing

被引：0
|
作者：
Benjamin J. Wainwright
Irma S. Arlyza
Stephen A. Karl
机构：
[1] University of Hawai‘i,Hawai‘i Institute of Marine Biology
[2] Mānoa,Research Center for Oceanography
[3] Indonesian Institute of Sciences,undefined
来源：
Conservation Genetics Resources | 2013年 / 5卷
关键词：
Connectivity; Gene flow; Indonesia; Conservation;
D O I：
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中图分类号：
学科分类号：
摘要：
Twenty-one microsatellite loci were developed for the tropical ascidian, Polycarpa aurata. Microsatellite enriched genomic libraries were constructed and sequenced using Pacific Biosciences single molecule real time sequencing technology. The 21 loci were characterized in 20 individuals from Alor, Indonesia. Observed levels of heterozygosity ranged from 0.30 to 1.00 with a mean of 8.2 alleles per locus. Loci PA21 and PA45 showed evidence of significant linkage disequilibrium and three loci significantly deviated from Hardy–Weinberg expectations.
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页码：671 / 673
页数：2
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