PCR-DGGE analysis of bacterial population attached to the bovine rumen wall

被引：0
|
作者：
F. Lukáš
J. Šimůnek
J. Mrázek
J. Kopečný
机构：
[1] Academy of Science of the Czech Republic,Institute of Animal Physiology and Genetics, v.v.i.
来源：
Folia Microbiologica | 2010年 / 55卷
关键词：
Bacterial Population; Lactococcus Lactis; Uncultured Bacterium; Rumen Content; Bovine Rumen;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken from each animal. The DGGE profile of the bacterial population attached to the rumen wall represented a high inter-animal variation.
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页码：345 / 348
页数：3
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