PCR-DGGE Analysis of Bacterial Population Attached to the Bovine Rumen Wall

被引：8
|
作者：
Lukas, F. [1 ]
Simunek, J. [1 ]
Mrazek, J. [1 ]
Kopecny, J. [1 ]
机构：
[1] Acad Sci Czech Republ, Inst Anim Physiol & Genet, Vvi, Prague 14220, Czech Republic
来源：
FOLIA MICROBIOLOGICA | 2010年 / 55卷 / 04期
关键词：
GRADIENT GEL-ELECTROPHORESIS; DIVERSITY; ADHERENT; EPITHELIUM; TRACT;
D O I：
10.1007/s12223-010-0055-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken from each animal. The DGGE profile of the bacterial population attached to the rumen wall represented a high inter-animal variation.
引用
收藏
页码：345 / 348
页数：4
相关论文
共 50 条
  • [1] PCR-DGGE analysis of bacterial population attached to the bovine rumen wall
    F. Lukáš
    J. Šimůnek
    J. Mrázek
    J. Kopečný
    [J]. Folia Microbiologica, 2010, 55 : 345 - 348
  • [2] PCR-DGGE analysis reveals a distinct diversity in the bacterial population attached to the rumen epithelium
    Sadet, S.
    Martin, C.
    Meunier, B.
    Morgavi, D. P.
    [J]. ANIMAL, 2007, 1 (07) : 939 - 944
  • [3] Diversity of bacterial population of table olives assessed by PCR-DGGE analysis
    Randazzo, Cinzia L.
    Ribbera, Angela
    Pitino, Iole
    Romeo, Flora V.
    Caggia, Cinzia
    [J]. FOOD MICROBIOLOGY, 2012, 32 (01) : 87 - 96
  • [4] Bacterial and Fungal Communities of Gioddu as Revealed by PCR-DGGE Analysis
    Maoloni, Antonietta
    Milanovic, Vesna
    Cardinali, Federica
    Mangia, Nicoletta P.
    Murgia, Marco A.
    Garofalo, Cristiana
    Clementi, Francesca
    Osimani, Andrea
    Aquilanti, Lucia
    [J]. INDIAN JOURNAL OF MICROBIOLOGY, 2020, 60 (01) : 119 - 123
  • [5] Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method
    Kuang, Ying
    Tani, Kaori
    Synnott, Aidan J.
    Ohshima, Kazuhito
    Higuchi, Hidetoshi
    Nagahata, Hajime
    Tanji, Yasunori
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2009, 45 (01) : 76 - 81
  • [6] Analysis of bacterial communities in soil by PCR-DGGE targeting protease genes
    Sakurai, Michihiko
    Suzuki, Katsumasa
    Onodera, Masayuki
    Shinano, Takuro
    Osaki, Mitsuru
    [J]. SOIL BIOLOGY & BIOCHEMISTRY, 2007, 39 (11): : 2777 - 2784
  • [7] Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis
    Fasoli, S
    Marzotto, M
    Rizzotti, L
    Rossi, F
    Dellaglio, F
    Torriani, S
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 82 (01) : 59 - 70
  • [8] PCR-DGGE Analysis of Bacterial Flora Changes in Processing RTE Abalone
    Zheng, Ruisheng
    Wu, Yinfeng
    Zhao, Yunfeng
    Wang, Qiaoyan
    Yang, Guiren
    Zheng, Zongping
    [J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21 (08) : 331 - 338
  • [9] PCR-DGGE Analysis of Bacterial Diversity of Intestinal System in Hyperlipidemia Rats
    Wu, Rina
    Pang, Xiaomeng
    Wang, Xiyan
    Wu, Junrui
    [J]. ADVANCES IN ENVIRONMENTAL TECHNOLOGIES, PTS 1-6, 2013, 726-731 : 898 - +
  • [10] PCR-DGGE ANALYSIS OF THE YEAST POPULATION ASSOCIATED WITH NATURAL FERMENTATION OF TABERNA
    Santiago-Urbina, Jorge A.
    Pena-Montes, Carolina
    Nolasco-Cancino, Hipocrates
    Ruiz-Teran, Francisco
    [J]. JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES, 2016, 6 (02): : 758 - 763
12345