PCR-DGGE Analysis of Bacterial Diversity of Intestinal System in Hyperlipidemia Rats

被引：0

作者：

Wu, Rina ^{[1
]}

Pang, Xiaomeng ^{[2
]}

Wang, Xiyan ^{[3
]}

Wu, Junrui ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Food Sci, Shenyang 110866, Peoples R China

[2] Shenyang Agr Univ, Minist Org, Shenyang 110866, Peoples R China

[3] Shenyang Agr Univ, Sch Vocat Technol, Shenyang 110866, Peoples R China

来源：

ADVANCES IN ENVIRONMENTAL TECHNOLOGIES, PTS 1-6 | 2013年 / 726-731卷

基金：

国家高技术研究发展计划(863计划);

关键词：

DGGE; hyperlipidemia; intestinal flora; GRADIENT GEL-ELECTROPHORESIS;

D O I：

10.4028/www.scientific.net/AMR.726-731.898

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene has been regarded as one of powerful tools for gaining insight into the bacterial diversity of intestinal system. In the present study, hyperlipidemia model was constructed in rat according to the tests of blood lipids. Fecal samples of rats were collected after 60d feeding, and DGGE was used to investigate the diversities of intestinal bacteria in the artificially-induced hyperlipidemia rats and normal rats. The results showed that two patterns had similarities, but there were also some different bacteria communities. Moreover, control group had much more bands than model group on gel, showing species in intestinal of model rats might be deduced by hyperlipidemia. It will be helpful to explore the relationship between hyperlipidemia and intestinal flora.

引用

页码：898 / +

页数：2

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