Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis

被引：1
|
作者：
Yann Guémas
Mohamed Boujtita
Nabil El Murr
机构：
[1] Faculté des Sciences et des Techniques,Groupe Électrochimie—Laboratoire d’Analyse Isotopique et Électrochimique de Métabolismes (UPRSA
来源：
Applied Biochemistry and Biotechnology | 2000年 / 89卷
关键词：
Biosensors; food analysis; glucose; sucrose; carbon paste electrode;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with <3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruitjuices. The results were also compared with those obtained with the ultraviolet method.
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页码：171 / 181
页数：10
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