Universal sample preparation method for proteome analysis

被引：0

作者：

Wiśniewski J.R. ^{[1
]}

Zougman A. ^{[1
]}

Nagaraj N. ^{[1
]}

Mann M. ^{[1
]}

机构：

[1] Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried

来源：

Nature Methods | 2009年 / 6卷 / 5期

关键词：

D O I：

10.1038/nmeth.1322

中图分类号：

学科分类号：

摘要：

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

引用

页码：359 / 362

页数：3

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