Universal sample preparation method for proteome analysis

被引：0
|
作者：
Wiśniewski J.R. [1 ]
Zougman A. [1 ]
Nagaraj N. [1 ]
Mann M. [1 ]
机构：
[1] Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried
来源：
Nature Methods | 2009年 / 6卷 / 5期
关键词：
D O I：
10.1038/nmeth.1322
中图分类号：
学科分类号：
摘要：
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
引用
收藏
页码：359 / 362
页数：3
相关论文
共 50 条
  • [1] Universal sample preparation method for proteome analysis
    Wisniewski, Jacek R.
    Zougman, Alexandre
    Nagaraj, Nagarjuna
    Mann, Matthias
    [J]. NATURE METHODS, 2009, 6 (05) : 359 - U60
  • [2] Sample preparation method for plasma membrane proteome analysis
    Park, Seung-Ah
    Kim, Mi-Ryung
    Kim, Pan-Kyeom
    Cho, Hyung-Don
    Han, Gi-Yeon
    Kim, Chan-Wha
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2008, 872 (1-2): : 177 - 180
  • [3] Reproducible sample preparation:: A key step in proteome analysis
    Hughes, KA
    Andrecht, S
    Abdolzade-Bavil, A
    Börner, A
    Leiss, D
    Rüter, C
    Hendriks, R
    Anders, J
    [J]. PROTEIN SCIENCE, 2004, 13 : 70 - 71
  • [4] 96DRA-Urine: A high throughput sample preparation method for urinary proteome analysis
    Tang, Xiaoyue
    Xiao, Xiaoping
    Sun, Haidan
    Zheng, Shuxin
    Xiao, Xiaolian
    Guo, Zhengguang
    Liu, Xiaoyan
    Sun, Wei
    [J]. JOURNAL OF PROTEOMICS, 2022, 257
  • [5] Reversible Click Chemistry Tag for Universal Proteome Sample Preparation for Top-Down and Bottom-Up Analysis
    Biedka, Stephanie
    Schmidt, Brigitte F.
    Frey, Nolan M.
    Boothman, Sarah M.
    Minden, Jonathan S.
    Lucas, Amber
    [J]. JOURNAL OF PROTEOME RESEARCH, 2021, 20 (10) : 4787 - 4800
  • [6] Evaluation of two sample preparation methods for prostate proteome analysis
    Lexander, Helena
    Hellman, Ulf
    Palmberg, Carina
    Auer, Gert
    Hellstrom, Magnus
    Franzen, Bo
    Jornvall, Hans
    Egevad, Lars
    [J]. PROTEOMICS, 2006, 6 (13) : 3918 - 3925
  • [7] An ionic liquid-assisted sample preparation method for sensitive integral-membrane proteome analysis
    Taoka, Masato
    Kamei, Kota
    Kashima, Akiko
    Nobe, Yuko
    Takekiyo, Takahiro
    Uekita, Takamasa
    Ichimura, Tohru
    [J]. ANALYTICAL BIOCHEMISTRY, 2023, 683
  • [8] Tips And Tricks for Proteome Sample Preparation
    Doucette, Alan A.
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2023, (192):
  • [9] UNIVERSAL METHOD OF SAMPLE PREPARATION FOR SPECTRAL-ISOTOPIC ANALYSIS OF NITROGEN IN NATURAL BIOLOGICAL SUBJECTS
    LASEJEVA, GS
    PETROV, AA
    KHOMJAKOV, RV
    [J]. VESTNIK LENINGRADSKOGO UNIVERSITETA SERIYA FIZIKA KHIMIYA, 1976, (04): : 153 - 155
  • [10] Strategies in sample preparation for proteome analysis of biological fluids from water buffaloes
    Balhara, Ashok Kumar
    Sangwan, Suman
    Ghosh, Mayukh
    Kumar, Rajesh
    Phulia, S. K.
    Singh, Inderjeet
    [J]. INDIAN JOURNAL OF ANIMAL SCIENCES, 2023, 93 (12): : 1174 - 1179
12345