An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels

被引：0

作者：

D. Bisht

N. Singhal

P. Sharma

K. Venkatesan

机构：

[1] National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research),Department of Biochemistry

来源：

Biochemistry (Moscow) | 2007年 / 72卷

关键词：

mycobacteria; tuberculosis; protein precipitation; two-dimensional gel electrophoresis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern.

引用

页码：672 / 674

页数：2

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