An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels

被引：0
|
作者：
D. Bisht
N. Singhal
P. Sharma
K. Venkatesan
机构：
[1] National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research),Department of Biochemistry
来源：
Biochemistry (Moscow) | 2007年 / 72卷
关键词：
mycobacteria; tuberculosis; protein precipitation; two-dimensional gel electrophoresis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern.
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页码：672 / 674
页数：2
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