An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels

被引：0
|
作者：
D. Bisht
N. Singhal
P. Sharma
K. Venkatesan
机构：
[1] National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research),Department of Biochemistry
来源：
Biochemistry (Moscow) | 2007年 / 72卷
关键词：
mycobacteria; tuberculosis; protein precipitation; two-dimensional gel electrophoresis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern.
引用
收藏
页码：672 / 674
页数：2
相关论文
共 50 条
  • [1] An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels
    Bisht, D.
    Singhal, N.
    Sharma, P.
    Venkatesan, K.
    BIOCHEMISTRY-MOSCOW, 2007, 72 (06) : 672 - 674
  • [2] A SIMPLE METHOD OF ELUTING PROTEINS FROM TWO-DIMENSIONAL GELS
    GUEVARA, J
    CHIOCCA, EA
    CLAYTON, FC
    VONESCHENBACH, AC
    EDWARDS, JJ
    CLINICAL CHEMISTRY, 1982, 28 (04) : 756 - 758
  • [3] Two-Dimensional Gels: An Easy Method for Large Quantities of Proteins
    Barent, Richard L.
    Elthon, Thomas E.
    PLANT MOLECULAR BIOLOGY REPORTER, 1992, 10 (04) : 338 - 344
  • [4] Improved resolution of human cerebrospinal fluid proteins on two-dimensional gels
    Hammack, BN
    Owens, GP
    Burgoon, MP
    Gilden, DH
    MULTIPLE SCLEROSIS, 2003, 9 (05): : 472 - 475
  • [5] A method of improving the separation of serum proteins on two-dimensional electrophoresis gels
    Zhong, X. L.
    Liu, X. H.
    Qian, L. J.
    Lv, Z. P.
    MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (10) : S234 - S234
  • [6] Two-dimensional gel electrophoresis of peripheral nerve proteins: Optimized sample preparation
    Qualtieri, Antonio
    Le Pera, Maria
    Urso, Elena
    Bono, Francesco
    Valentino, Paola
    Scornaienchi, Massimo Corrado
    Quattrone, Aldo
    JOURNAL OF NEUROSCIENCE METHODS, 2007, 159 (01) : 125 - 133
  • [7] Rapid identification of yeast proteins on two-dimensional gels
    Maillet, I
    Lagniel, G
    Perrot, M
    Boucherie, H
    Labarre, J
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (17) : 10263 - 10270
  • [8] Sample preparation for two-dimensional gel electrophoresis
    Shaw, MM
    Riederer, BM
    PROTEOMICS, 2003, 3 (08) : 1408 - 1417
  • [9] Sample preparation guidelines for two-dimensional electrophoresis
    Posch, Anton
    ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, 2014, 120 (05) : 192 - 197
  • [10] PREPARATION OF CHLOROPLAST PROTEINS FOR TWO-DIMENSIONAL ELECTROPHORESIS
    PORTER, MA
    PAULSEN, GM
    REECK, GR
    PHOTOSYNTHETICA, 1985, 19 (04) : 514 - 519
12345