Light-induced protein degradation in human-derived cells

被引：14
|
作者：
Sun, Wansheng [1 ]
Zhang, Wenyao [1 ,2 ]
Zhang, Chao [1 ]
Mao, Miaowei [1 ,2 ]
Zhao, Yuzheng [1 ,2 ]
Chen, Xianjun [1 ,2 ]
Yang, Yi [1 ,2 ]
机构：
[1] East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai 200237, Peoples R China
[2] East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 487卷 / 02期
基金：
中国博士后科学基金;
关键词：
Light; Protein degradation; Light-switchable degron; GENE-EXPRESSION; LIVING CELLS; SYSTEM; ACTIVATION; PROTEASOME; INDUCTION; STABILITY; DOMAIN; MICE;
D O I：
10.1016/j.bbrc.2017.04.041
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin I (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：241 / 246
页数：6
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