Light-induced protein degradation in human-derived cells

被引：14

作者：

Sun, Wansheng ^{[1
]}

Zhang, Wenyao ^{[1
,2
]}

Zhang, Chao ^{[1
]}

Mao, Miaowei ^{[1
,2
]}

Zhao, Yuzheng ^{[1
,2
]}

Chen, Xianjun ^{[1
,2
]}

Yang, Yi ^{[1
,2
]}

机构：

[1] East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai 200237, Peoples R China

[2] East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 487卷 / 02期

基金：

中国博士后科学基金;

关键词：

Light; Protein degradation; Light-switchable degron; GENE-EXPRESSION; LIVING CELLS; SYSTEM; ACTIVATION; PROTEASOME; INDUCTION; STABILITY; DOMAIN; MICE;

D O I：

10.1016/j.bbrc.2017.04.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin I (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：241 / 246

页数：6

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