Ambiguous base pairing of 1-(2-deoxy-beta-D-ribofuranosyl) imidazole-4-carboxamide during PCR

被引：2
|
作者：
Pochet, S [1 ]
Dugue, L [1 ]
Sala, M [1 ]
Pezo, V [1 ]
WainHobson, S [1 ]
机构：
[1] INST PASTEUR,UNITE RETROVIROL MOL,F-75724 PARIS,FRANCE
来源：
NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 7-9期
关键词：
D O I：
10.1080/07328319708006269
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of 5'-triphosphate of 1-(2-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide (dYTP) in DNA amplification reaction in place of dATP or dGTP yielded mutations frequencies of 3-4x10(-2) per base per amplification. A reasonable proportion of transversions (11-15%) was observed in the absence of deletions and insertions.
引用
收藏
页码：1749 / 1752
页数：4
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