CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells

被引：5
|
作者：
Tomizawa, Minoru [1 ]
Shinozaki, Fuminobu [2 ]
Motoyoshi, Yasufumi [3 ]
Sugiyama, Takao [4 ]
Yamamoto, Shigenori [5 ]
Ishige, Naoki [6 ]
机构：
[1] Shimoshizu Hosp, Natl Hosp Org, Dept Gastroenterol, 934-5 Shikawatashi, Chiba 2840003, Japan
[2] Shimoshizu Hosp, Natl Hosp Org, Dept Radiol, Chiba 2840003, Japan
[3] Shimoshizu Hosp, Natl Hosp Org, Dept Neurol, Chiba 2840003, Japan
[4] Shimoshizu Hosp, Natl Hosp Org, Dept Rheumatol, Chiba 2840003, Japan
[5] Shimoshizu Hosp, Natl Hosp Org, Dept Pediat, Chiba 2840003, Japan
[6] Shimoshizu Hosp, Natl Hosp Org, Dept Neurosurg, Chiba 2840003, Japan
来源：
ONCOLOGY LETTERS | 2017年 / 13卷 / 06期
基金：
日本学术振兴会;
关键词：
gastric cancer; CCAAT/enhancer-binding protein alpha; CCAAT/enhancer-binding protein beta; CCAAT/enhancer-binding protein delta; reverse transcription-quantitative polymerase chain reaction; 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium inner salt assay; episomal vector; EXPRESSION; GENE; HEPATOCYTE; DELTA; BETA;
D O I：
10.3892/ol.2017.5987
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
CCAAT/enhancer-binding protein (C/EBP) alpha, C/EBP beta and C/EBP delta are involved in inflammation and cell differentiation. In the present study, their roles in human gastric cancer cells were investigated. The human gastric cancer cell lines MKN45 and MKN74 were subjected to the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the expression levels of C/EBP alpha, C/EBP beta and C/EBPd. The cells were transfected with expression plasmids for either C/EBP alpha or C/EBP delta, and subjected to a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium inner salt (MTS) assay and RT-qPCR for analysis of cyclin D1 expression. Expression levels of C/EBP alpha and C/EBP delta were decreased in MKN45 and MKN74 cells compared with in normal gastric tissue. Expression levels of C/EBP beta were decreased in MKN45 cells and increased in MKN74 cells. Viability of MKN45 cells was decreased by C/EBP alpha and C/EBP delta. Viability of MKN74 cells was decreased by C/EBP alpha, but increased by C/EBPd. Expression levels of cyclin D1 were decreased in association with C/EBP alpha and C/EBP delta overexpression in MKN45 cells. Expression levels of cyclin D1 were decreased in association with C/EBP alpha overexpression, but increased in association with C/EBP delta overexpression, in MKN74 cells. The results of the present study indicate that C/EBP alpha is potentially useful for the treatment of gastric cancer.
引用
收藏
页码：4322 / 4326
页数：5
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