CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells

被引：5
|
作者：
Tomizawa, Minoru [1 ]
Shinozaki, Fuminobu [2 ]
Motoyoshi, Yasufumi [3 ]
Sugiyama, Takao [4 ]
Yamamoto, Shigenori [5 ]
Ishige, Naoki [6 ]
机构：
[1] Shimoshizu Hosp, Natl Hosp Org, Dept Gastroenterol, 934-5 Shikawatashi, Chiba 2840003, Japan
[2] Shimoshizu Hosp, Natl Hosp Org, Dept Radiol, Chiba 2840003, Japan
[3] Shimoshizu Hosp, Natl Hosp Org, Dept Neurol, Chiba 2840003, Japan
[4] Shimoshizu Hosp, Natl Hosp Org, Dept Rheumatol, Chiba 2840003, Japan
[5] Shimoshizu Hosp, Natl Hosp Org, Dept Pediat, Chiba 2840003, Japan
[6] Shimoshizu Hosp, Natl Hosp Org, Dept Neurosurg, Chiba 2840003, Japan
来源：
ONCOLOGY LETTERS | 2017年 / 13卷 / 06期
基金：
日本学术振兴会;
关键词：
gastric cancer; CCAAT/enhancer-binding protein alpha; CCAAT/enhancer-binding protein beta; CCAAT/enhancer-binding protein delta; reverse transcription-quantitative polymerase chain reaction; 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium inner salt assay; episomal vector; EXPRESSION; GENE; HEPATOCYTE; DELTA; BETA;
D O I：
10.3892/ol.2017.5987
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
CCAAT/enhancer-binding protein (C/EBP) alpha, C/EBP beta and C/EBP delta are involved in inflammation and cell differentiation. In the present study, their roles in human gastric cancer cells were investigated. The human gastric cancer cell lines MKN45 and MKN74 were subjected to the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the expression levels of C/EBP alpha, C/EBP beta and C/EBPd. The cells were transfected with expression plasmids for either C/EBP alpha or C/EBP delta, and subjected to a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium inner salt (MTS) assay and RT-qPCR for analysis of cyclin D1 expression. Expression levels of C/EBP alpha and C/EBP delta were decreased in MKN45 and MKN74 cells compared with in normal gastric tissue. Expression levels of C/EBP beta were decreased in MKN45 cells and increased in MKN74 cells. Viability of MKN45 cells was decreased by C/EBP alpha and C/EBP delta. Viability of MKN74 cells was decreased by C/EBP alpha, but increased by C/EBPd. Expression levels of cyclin D1 were decreased in association with C/EBP alpha and C/EBP delta overexpression in MKN45 cells. Expression levels of cyclin D1 were decreased in association with C/EBP alpha overexpression, but increased in association with C/EBP delta overexpression, in MKN74 cells. The results of the present study indicate that C/EBP alpha is potentially useful for the treatment of gastric cancer.
引用
收藏
页码：4322 / 4326
页数：5
相关论文
共 50 条
  • [31] CCAAT/Enhancer-Binding Protein β (C/EBPβ) Regulates Calcium Deposition in Smooth Muscle Cells
    Choe, Nakwon
    Shin, Sera
    Kim, Young-Kook
    Kook, Hyun
    Kwon, Duk-Hwa
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (24)
  • [32] Complex regulation of the fibroblast growth factor-binding protein in MDAMB-468 breast cancer cells by CCAAT/enhancer-binding protein β
    Kagan, BL
    Henke, RT
    Cabal-Manzano, R
    Stoica, GE
    Nguyen, Q
    Wellstein, A
    Riegel, AT
    CANCER RESEARCH, 2003, 63 (07) : 1696 - 1705
  • [33] Identification of functional CCAAT/enhancer-binding protein and Ets protein binding sites in the human chorionic somatomammotropin enhancer sequences
    Lytras, Aristides
    Detillieux, Karen
    Cattini, Peter A.
    JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2011, 47 (02) : 179 - 193
  • [34] Interleukin-8 Overexpression in Astrocytomas Is Induced by Prostaglandin E2 and Is Associated With the Transcription Factors CCAAT/Enhancer-Binding Protein-β and CCAAT/Enhancer-Binding Homologous Protein
    Venza, Mario
    Visalli, Maria
    Alafaci, Concetta
    Caffo, Maria
    Caruso, Gerardo
    Salpietro, Francesco Maria
    Tomasello, Francesco
    Teti, Diana
    NEUROSURGERY, 2011, 69 (03) : 713 - 721
  • [35] Regulation of CCAAT/Enhancer-binding Protein Homologous Protein (CHOP) Expression by Interleukin-1β in Pancreatic β Cells
    Shao, Chunli
    Lawrence, Michael C.
    Cobb, Melanie H.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (26) : 19710 - 19719
  • [36] INSULIN INHIBITS LIVER EXPRESSION OF THE CCAAT/ENHANCER-BINDING PROTEIN-BETA
    BOSCH, F
    SABATER, J
    VALERA, A
    DIABETES, 1995, 44 (03) : 267 - 271
  • [37] The CCAAT/Enhancer-Binding Protein Family: Its Roles in MDSC Expansion and Function
    Wang, Wenxin
    Xia, Xueli
    Mao, Lingxiang
    Wang, Shengjun
    FRONTIERS IN IMMUNOLOGY, 2019, 10 : 1804
  • [38] Inhibition of CCAAT/enhancer-binding protein α and β translation by upstream open reading frames
    Lincoln, AJ
    Monczak, Y
    Williams, SC
    Johnson, PF
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (16) : 9552 - 9560
  • [39] Physical dissection of the CCAAT/enhancer-binding protein α in regulating the mouse amelogenin gene
    Xu, Yucheng
    Zhou, Yan Larry
    Erickson, Robin L.
    MacDougald, Ormond A.
    Snead, Malcolm L.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 354 (01) : 56 - 61
  • [40] Action and clinical significance of CCAAT/enhancer-binding protein delta in hepatocellular carcinoma
    Liu, Pengyu
    Cao, Wanlu
    Ma, Buyun
    Li, Meng
    Chen, Kan
    Sideras, Kostandinos
    Duitman, Jan-Willem
    Sprengers, Dave
    Tran, T. C. Khe
    Ijzermans, Jan N. M.
    Biermann, Katharina
    Verheij, Joanne
    Spek, C. Arnold
    Kwekkeboom, Jaap
    Pan, Qiuwei
    Peppelenbosch, Maikel P.
    CARCINOGENESIS, 2019, 40 (01) : 155 - 163
12345