Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens

被引:12
|
作者
Fernandes, Tomas M. [1 ]
Morgado, Leonor [1 ]
Salgueiro, Carlos A. [1 ]
机构
[1] Univ Nova Lisboa, UCIBIO Requimte, Dept Quim, Fac Ciencias & Tecnol, Campus Caparica, P-2829516 Caparica, Portugal
关键词
C-TYPE CYTOCHROME; DISSIMILATORY METAL REDUCTION; FE(III) OXIDE; ELECTROCHEMICAL CHARACTERIZATION; DESULFUROMONAS ACETOXIDANS; CONTAMINATED GROUNDWATER; REDUCING MICROORGANISM; DESULFOVIBRIO-VULGARIS; AROMATIC-HYDROCARBONS; ANAEROBIC DEGRADATION;
D O I
10.1042/BCJ20180457
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Geobacter metallireducens bacterium can couple the oxidation of a wide range of compounds to the reduction of several extracellular electron acceptors, including pollutants or electrode surfaces for current production in microbial fuel cells. For these reasons, G. metallireducens are of interest for practical biotechnological applications. The use of such electron acceptors relies on a mechanism that permits electrons to be transferred to the cell exterior. The cytochrome PpcA from G. metallireducens is a member of a family composed of five periplasmic triheme cytochromes, which are important to bridge the electron transfer from the cytoplasmic donors to the extracellular acceptors. Using NMR and visible spectroscopic techniques, a detailed thermodynamic characterization of PpcA was obtained, including the determination of the heme reduction potentials and their redox and redox-Bohr interactions. These parameters revealed unique features for PpcA from G. metallireducens compared with other triheme cytochromes from different microorganisms, namely the less negative heme reduction potentials and concomitant functional working potential ranges. It was also shown that the order of oxidation of the hemes is pH-independent, but the protein is designed to couple e(-)/H+ transfer exclusively at physiological pH.
引用
收藏
页码:2861 / 2875
页数:15
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