Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens

被引:12
|
作者
Fernandes, Tomas M. [1 ]
Morgado, Leonor [1 ]
Salgueiro, Carlos A. [1 ]
机构
[1] Univ Nova Lisboa, UCIBIO Requimte, Dept Quim, Fac Ciencias & Tecnol, Campus Caparica, P-2829516 Caparica, Portugal
关键词
C-TYPE CYTOCHROME; DISSIMILATORY METAL REDUCTION; FE(III) OXIDE; ELECTROCHEMICAL CHARACTERIZATION; DESULFUROMONAS ACETOXIDANS; CONTAMINATED GROUNDWATER; REDUCING MICROORGANISM; DESULFOVIBRIO-VULGARIS; AROMATIC-HYDROCARBONS; ANAEROBIC DEGRADATION;
D O I
10.1042/BCJ20180457
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Geobacter metallireducens bacterium can couple the oxidation of a wide range of compounds to the reduction of several extracellular electron acceptors, including pollutants or electrode surfaces for current production in microbial fuel cells. For these reasons, G. metallireducens are of interest for practical biotechnological applications. The use of such electron acceptors relies on a mechanism that permits electrons to be transferred to the cell exterior. The cytochrome PpcA from G. metallireducens is a member of a family composed of five periplasmic triheme cytochromes, which are important to bridge the electron transfer from the cytoplasmic donors to the extracellular acceptors. Using NMR and visible spectroscopic techniques, a detailed thermodynamic characterization of PpcA was obtained, including the determination of the heme reduction potentials and their redox and redox-Bohr interactions. These parameters revealed unique features for PpcA from G. metallireducens compared with other triheme cytochromes from different microorganisms, namely the less negative heme reduction potentials and concomitant functional working potential ranges. It was also shown that the order of oxidation of the hemes is pH-independent, but the protein is designed to couple e(-)/H+ transfer exclusively at physiological pH.
引用
收藏
页码:2861 / 2875
页数:15
相关论文
共 50 条
  • [31] The robustness of porin-cytochrome gene clusters from Geobacter metallireducens in extracellular electron transfer
    Zhuo, Shiyan
    Jiang, Yongguang
    Qi, Lei
    Hu, Yidan
    Jiang, Zhou
    Dong, Yiran
    Shi, Liang
    MBIO, 2024, 15 (09):
  • [32] An unusual active site architecture in cytochrome c nitrite reductase NrfA-1 from Geobacter metallireducens
    Denkhaus, Lukas
    Siffert, Fanny
    Einsle, Oliver
    FEMS MICROBIOLOGY LETTERS, 2023, 370
  • [33] Role of Met58 in the regulation of electron/proton transfer in trihaem cytochrome PpcA from Geobacter sulfurreducens
    Morgado, Leonor
    Dantas, Joana M.
    Simoes, Telma
    Londer, Yuri Y.
    Raj Pokkuluri, P.
    Salgueiro, Carlos A.
    BIOSCIENCE REPORTS, 2013, 33 : 11 - U28
  • [34] Structural and functional characterization of the unusual triheme cytochrome bound to the reaction center of Rhodovulum sulfidophilum
    Alric, J
    Tsukatani, Y
    Yoshida, M
    Matsuura, K
    Shimada, K
    Hienerwadel, R
    Schoepp-Cothenet, B
    Nitschke, W
    Nagashima, KVP
    Verméglio, A
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (25) : 26090 - 26097
  • [35] Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens
    Dantas, Joana M.
    Tomaz, Diogo M.
    Morgado, Leonor
    Salgueiro, Carlos A.
    FEBS LETTERS, 2013, 587 (16) : 2662 - 2668
  • [36] Structure Determination and Biochemical Characterization of a Putative HNH Endonuclease from Geobacter metallireducens GS-15
    Xu, Shuang-yong
    Kuzin, Alexandre P.
    Seetharaman, Jayaraman
    Gutjahr, Alice
    Chan, Siu-Hong
    Chen, Yang
    Xiao, Rong
    Acton, Thomas B.
    Montelione, Gaetano T.
    Tong, Liang
    PLOS ONE, 2013, 8 (09):
  • [37] Biomolecular Interaction Studies Between Cytochrome PpcA From Geobacter sulfurreducens and the Electron Acceptor Ferric Nitrilotriacetate (Fe-NTA)
    Ferreira, Marisa R.
    Salgueiro, Carlos A.
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [38] Dissecting the Functional Role of Key Residues in Triheme Cytochrome PpcA: A Path to Rational Design of G-sulfurreducens Strains with Enhanced Electron Transfer Capabilities
    Morgado, Leonor
    Lourenco, Silvia
    Londer, Yuri Y.
    Schiffer, Marianne
    Pokkuluri, P. Raj
    Salgueiro, Carlos A.
    PLOS ONE, 2014, 9 (08):
  • [39] Electron Paramagnetic Resonance Characterization of the Triheme Cytochrome from &ITGeobacter sulfurreducens&IT
    Ponomarenko, Nina
    Niklas, Jens
    Pokkuluri, P. Raj
    Poluektov, Oleg
    Tiede, David M.
    BIOCHEMISTRY, 2018, 57 (11) : 1722 - 1732
  • [40] Structural and Functional Insights of an Unprecedented Multiheme Cytochrome from Geobacter Sulfurreducens
    Fernandes, Tomas M.
    Morgado, Leonor
    Folgosa, Filipe
    Teixeira, Miguel
    Salgueiro, Carlos A.
    BIOPHYSICAL JOURNAL, 2021, 120 (03) : 119A - 119A