Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay

被引：11
|
作者：
Ahn, Jong Hoon [1 ]
Jo, Yang Hee [1 ]
Kim, Seon Beom [1 ]
Turk, Ayman [1 ]
Oh, Kyung-Eon [2 ]
Hwang, Bang Yeon [1 ]
Lee, Ki Yong [2 ]
Lee, Mi Kyeong [1 ]
机构：
[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 28160, South Korea
[2] Korea Univ, Coll Pharm, Sejong 30019, South Korea
来源：
PHYTOCHEMISTRY LETTERS | 2018年 / 26卷
基金：
新加坡国家研究基金会;
关键词：
Plantago asiatica; Plantaginaceae; phenylethanoid; LC-MS/MS online assay; Iridoid; OXIDATIVE STRESS; GLYCOSIDES;
D O I：
10.1016/j.phytol.2018.05.006
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Screening of EtOAc soluble fraction of Plantago asiatica (Plantaginaceae) using HPLC-MS/MS coupled DPPH online assay suggested the presence of antioxidant compounds, which characterized as phenylethanoid glycosides. Twenty-nine compounds including three new compounds, plantalides A-C, a phenylethanoid (1), a phenolic compound (9) and an iridoid (10), were isolated from P. asiatica. Consistent with HPLC-MS/MS analysis, seven phenylethanoid glycosides (1-7) including a new phenylethanoid glycoside (1) showed antioxidant with ranging IC50 values of 22.9-88.5 mu M. These results suggested that phenylethanoid glycosides were contributed to the antioxidant activity of P. asiatica and HPLC-MS/MS coupled online assay is powerful tool for predicting active compounds with short time and limited amounts.
引用
收藏
页码：20 / 24
页数：5
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