Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay

被引：11

作者：

Ahn, Jong Hoon ^{[1
]}

Jo, Yang Hee ^{[1
]}

Kim, Seon Beom ^{[1
]}

Turk, Ayman ^{[1
]}

Oh, Kyung-Eon ^{[2
]}

Hwang, Bang Yeon ^{[1
]}

Lee, Ki Yong ^{[2
]}

Lee, Mi Kyeong ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 28160, South Korea

[2] Korea Univ, Coll Pharm, Sejong 30019, South Korea

来源：

PHYTOCHEMISTRY LETTERS | 2018年 / 26卷

基金：

新加坡国家研究基金会;

关键词：

Plantago asiatica; Plantaginaceae; phenylethanoid; LC-MS/MS online assay; Iridoid; OXIDATIVE STRESS; GLYCOSIDES;

D O I：

10.1016/j.phytol.2018.05.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Screening of EtOAc soluble fraction of Plantago asiatica (Plantaginaceae) using HPLC-MS/MS coupled DPPH online assay suggested the presence of antioxidant compounds, which characterized as phenylethanoid glycosides. Twenty-nine compounds including three new compounds, plantalides A-C, a phenylethanoid (1), a phenolic compound (9) and an iridoid (10), were isolated from P. asiatica. Consistent with HPLC-MS/MS analysis, seven phenylethanoid glycosides (1-7) including a new phenylethanoid glycoside (1) showed antioxidant with ranging IC50 values of 22.9-88.5 mu M. These results suggested that phenylethanoid glycosides were contributed to the antioxidant activity of P. asiatica and HPLC-MS/MS coupled online assay is powerful tool for predicting active compounds with short time and limited amounts.

引用

页码：20 / 24

页数：5

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