Cloning and Characterisation of Nanobodies against the Coat Protein of Zucchini Yellow Mosaic Virus

被引：4
|
作者：
Zakri, Adel M. [1 ]
Al-Doss, Abdullah A. [1 ]
Sack, Markus [4 ]
Ali, Ahmed A. [1 ]
Samara, Emad M. [2 ]
Ahmed, Basem S. [1 ]
Amer, Mahmoud A. [3 ]
Abdalla, Omar A. [3 ]
Al-Saleh, Mohammed A. [3 ]
机构：
[1] King Saud Univ, Coll Food & Agr Sci, Dept Plant Prod, Riyadh, Saudi Arabia
[2] King Saud Univ, Coll Food & Agr Sci, Dept Anim Prod, Riyadh, Saudi Arabia
[3] King Saud Univ, Coll Food & Agr Sci, Dept Plant Protect, Riyadh, Saudi Arabia
[4] Rhein Westfal TH Aachen, Inst Mol Biotechnol, Aachen, Germany
来源：
PLANT PROTECTION SCIENCE | 2018年 / 54卷 / 04期
关键词：
nanobodies; VHH; naive library; phage display; SINGLE-DOMAIN ANTIBODIES; PHAGE DISPLAY LIBRARY; PLANT-VIRUS; RECOMBINANT ANTIBODIES; CAMELID NANOBODIES; CANCER-THERAPY; RESISTANCE; CHAIN; FRAGMENTS; INFECTION;
D O I：
10.17221/158/2017-PPS
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Zucchini yellow mosaic virus (ZYMV), in the family Potyviridae, causes an economically important disease. Antibodies are valuable reagents for diagnostic assays to rapidly detect viral infection. Here, we report the isolation of camel-derived variable domains of the heavy chain antibody (VHH, also called nanobodies) directed against the coat protein (CP) of ZYMV. Several nanobodies that specifically recognise ZYMV-CP were identified. The isolated nanobodies showed binding not only to recombinant ZYMV-CP but also to native ZYMV, indicating that these nanobodies can be used in diagnostic tools to detect viral infections.
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页码：215 / 221
页数：7
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