Cloning and Characterisation of Nanobodies against the Coat Protein of Zucchini Yellow Mosaic Virus

被引：4

作者：

Zakri, Adel M. ^{[1
]}

Al-Doss, Abdullah A. ^{[1
]}

Sack, Markus ^{[4
]}

Ali, Ahmed A. ^{[1
]}

Samara, Emad M. ^{[2
]}

Ahmed, Basem S. ^{[1
]}

Amer, Mahmoud A. ^{[3
]}

Abdalla, Omar A. ^{[3
]}

Al-Saleh, Mohammed A. ^{[3
]}

机构：

[1] King Saud Univ, Coll Food & Agr Sci, Dept Plant Prod, Riyadh, Saudi Arabia

[2] King Saud Univ, Coll Food & Agr Sci, Dept Anim Prod, Riyadh, Saudi Arabia

[3] King Saud Univ, Coll Food & Agr Sci, Dept Plant Protect, Riyadh, Saudi Arabia

[4] Rhein Westfal TH Aachen, Inst Mol Biotechnol, Aachen, Germany

来源：

PLANT PROTECTION SCIENCE | 2018年 / 54卷 / 04期

关键词：

nanobodies; VHH; naive library; phage display; SINGLE-DOMAIN ANTIBODIES; PHAGE DISPLAY LIBRARY; PLANT-VIRUS; RECOMBINANT ANTIBODIES; CAMELID NANOBODIES; CANCER-THERAPY; RESISTANCE; CHAIN; FRAGMENTS; INFECTION;

D O I：

10.17221/158/2017-PPS

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Zucchini yellow mosaic virus (ZYMV), in the family Potyviridae, causes an economically important disease. Antibodies are valuable reagents for diagnostic assays to rapidly detect viral infection. Here, we report the isolation of camel-derived variable domains of the heavy chain antibody (VHH, also called nanobodies) directed against the coat protein (CP) of ZYMV. Several nanobodies that specifically recognise ZYMV-CP were identified. The isolated nanobodies showed binding not only to recombinant ZYMV-CP but also to native ZYMV, indicating that these nanobodies can be used in diagnostic tools to detect viral infections.

引用

下载

页码：215 / 221

页数：7

共 50 条

[21] First Report of Zucchini yellow mosaic virus in Chrysanthemum
Niu, E. B.
Chen, L. J.
Niu, Y. B.
PLANT DISEASE, 2015, 99 (09) : 1289 - 1289
[22] INHERITANCE OF CUCUMBER TOLERANCE TO ZUCCHINI YELLOW MOSAIC VIRUS
Cardoso, A. I. I.
Pavan, M. A.
Sakate, R. Krause
Fattori, K.
JOURNAL OF PLANT PATHOLOGY, 2010, 92 (01) : 245 - 248
[23] Characterization and occurrence of Zucchini Yellow Mosaic Virus in Sudan
Mahgoub, HA
Desbiez, C
WipfScheibel, C
Dafalla, G
Lecoq, H
PLANT PATHOLOGY, 1997, 46 (05) : 800 - 805
[24] Inheritance of Resistance to Zucchini Yellow Mosaic Virus in Watermelon
Guner, Nihat
Rivera-Burgos, Luis A.
Wehner, Todd C.
HORTSCIENCE, 2018, 53 (08) : 1115 - 1118
[25] PURIFICATION AND CLONING OF ZUCCHINI YELLOWS MOSAIC-VIRUS
GRUMET, R
HAUFLER, KZ
HORTSCIENCE, 1988, 23 (03) : 755 - 755
[26] PRESENCE OF CUCURBIT VIRUSES IN ANKARA AND ANTALYA PROVINCE AND MOLECULAR CHARACTERIZATION OF COAT PROTEIN GENE OF ZUCCHINI YELLOW MOSAIC VIRUS TURKISH ISOLATES
Topkaya, Serife
Desbiez, Cecile
Ertunc, Filiz
FRESENIUS ENVIRONMENTAL BULLETIN, 2019, 28 (04): : 2442 - 2449
[27] Characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains
Desbiez, C
GalOn, A
Raccah, B
Lecoq, H
JOURNAL OF GENERAL VIROLOGY, 1997, 78 : 2073 - 2076
[28] Recombinant turnip yellow mosaic virus coat protein as a potential nanocarrier
Tan, F. H.
Kong, J. C.
Ng, J. F.
Alitheen, N. B.
Wong, C. L.
Yong, C. Y.
Lee, K. W.
JOURNAL OF APPLIED MICROBIOLOGY, 2021, 131 (04) : 2072 - 2080
[29] GENETIC-ENGINEERING OF POTYVIRUS RESISTANCE USING CONSTRUCTS DERIVED FROM THE ZUCCHINI YELLOW MOSAIC-VIRUS COAT PROTEIN GENE
FANG, GW
GRUMET, R
MOLECULAR PLANT-MICROBE INTERACTIONS, 1993, 6 (03) : 358 - 367
[30] Maintenance of coat protein N-terminal net charge and not primary sequence is essential for zucchini yellow mosaic virus systemic infectivity
Kimalov, B
Gal-On, A
Stav, R
Belausov, E
Arazi, T
JOURNAL OF GENERAL VIROLOGY, 2004, 85 : 3421 - 3430

← 1 2 3 4 5 →