The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase

被引：62
|
作者：
De Bie, LGS
Roovers, M
Oudjama, Y
Wattiez, R
Tricot, C
Stalon, V
Droogmans, L
Bujnicki, JM
机构：
[1] Free Univ Brussels, Microbiol Lab, Inst Rech Microbiol JM Wiame, B-1070 Brussels, Belgium
[2] Univ Mons, Chim Biol Lab, B-7000 Mons, Belgium
[3] Int Inst Mol & Cell Biol, Bioinformat Lab, PL-02109 Warsaw, Poland
来源：
JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 10期
关键词：
D O I：
10.1128/JB.185.10.3238-3243.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N-7-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.
引用
收藏
页码：3238 / 3243
页数：6
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