The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase

被引：62
|
作者：
De Bie, LGS
Roovers, M
Oudjama, Y
Wattiez, R
Tricot, C
Stalon, V
Droogmans, L
Bujnicki, JM
机构：
[1] Free Univ Brussels, Microbiol Lab, Inst Rech Microbiol JM Wiame, B-1070 Brussels, Belgium
[2] Univ Mons, Chim Biol Lab, B-7000 Mons, Belgium
[3] Int Inst Mol & Cell Biol, Bioinformat Lab, PL-02109 Warsaw, Poland
来源：
JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 10期
关键词：
D O I：
10.1128/JB.185.10.3238-3243.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N-7-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.
引用
收藏
页码：3238 / 3243
页数：6
相关论文
共 50 条
  • [21] ESCHERICHIA-COLI MUTY GENE ENCODES AN ADENINE GLYCOSYLASE ACTIVE ON G-A MISPAIRS
    AU, KG
    CLARK, S
    MILLER, JH
    MODRICH, P
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) : 8877 - 8881
  • [22] The C-terminal region of thermophilic tRNA (m7 G46) methyltransferase (TrmB) stabilizes the dimer structure and enhances fidelity of methylation
    Tomikawa, Chie
    Ochi, Anna
    Hori, Hiroyuki
    PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2008, 71 (03) : 1400 - 1408
  • [23] Crystal structure of tRNA(m1G37)methyltransferase:: insights into tRNA recognition
    Ahn, HJ
    Kim, HW
    Yoon, HJ
    Lee, BI
    Suh, SW
    Yang, JK
    EMBO JOURNAL, 2003, 22 (11): : 2593 - 2603
  • [24] Distinct origins of tRNA(M1G37) methyltransferase
    Christian, T
    Evilia, C
    Williams, S
    Hou, YM
    JOURNAL OF MOLECULAR BIOLOGY, 2004, 339 (04) : 707 - 719
  • [25] A novel pathogenicity island integrated adjacent to the thrW tRNA gene of avian pathogenic Escherichia coli encodes a vacuolating autotransporter toxin
    Parreira, VR
    Gyles, CL
    INFECTION AND IMMUNITY, 2003, 71 (09) : 5087 - 5096
  • [26] The gene yjcG, cotranscribed with the gene acs, encodes an acetate permease in Escherichia coli
    Gimenez, R
    Nuñez, MF
    Badia, J
    Aguilar, J
    Baldoma, L
    JOURNAL OF BACTERIOLOGY, 2003, 185 (21) : 6448 - 6455
  • [27] Amino acid residues of the Escherichia coli tRNA(m5U54) methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity
    Urbonavicius, Jaunius
    Jager, Gunilla
    Bjork, Glenn R.
    NUCLEIC ACIDS RESEARCH, 2007, 35 (10) : 3297 - 3305
  • [28] The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity
    Persson, BC
    Jager, G
    Gustafsson, C
    NUCLEIC ACIDS RESEARCH, 1997, 25 (20) : 4093 - 4097
  • [29] Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9
    Jackman, JE
    Montange, RK
    Malik, HS
    Phizicky, EM
    RNA, 2003, 9 (05) : 574 - 585
  • [30] Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA
    Wang, Kai-Tuo
    Desmolaize, Benoit
    Nan, Jie
    Zhang, Xiao-Wei
    Li, Lan-Fen
    Douthwaite, Stephen
    Su, Xiao-Dong
    NUCLEIC ACIDS RESEARCH, 2012, 40 (11) : 5138 - 5148
12345