Binding characteristics of SLE anti-DNA autoantibodies to modified DNA analogues

被引：0
|
作者：
Arjumand, S [1 ]
Moinuddin [1 ]
Ali, A [1 ]
机构：
[1] Aligarh Muslim Univ, Fac Med, Dept Biochem, Aligarh, Uttar Pradesh, India
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1997年 / 43卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti-DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity of anti-DNA antibodies for modified conformers was found to be quite high.
引用
收藏
页码：643 / 653
页数：11
相关论文
共 50 条
  • [31] THE ORIGIN AND PATHOGENIC ROLE OF ANTI-DNA AUTOANTIBODIES
    STOLLAR, BD
    CURRENT OPINION IN IMMUNOLOGY, 1990, 2 (04) : 607 - 612
  • [32] Binding of human anti-DNA autoantibodies to reactive oxygen species modified-DNA and probing oxidative DNA damage in cancer using monoclonal antibody
    Ashok, BT
    Ali, R
    INTERNATIONAL JOURNAL OF CANCER, 1998, 78 (04) : 404 - 409
  • [33] Ligand recognition by anti-DNA autoantibodies. Affinity, specificity, and mode of binding
    Swanson, PC
    Ackroyd, C
    Glick, GD
    BIOCHEMISTRY, 1996, 35 (05) : 1624 - 1633
  • [34] DNAse treatment of DNA - Anti-DNA immune complexes in SLE.
    Klinman, D
    Elias, K
    Shak, S
    Verthelyi, D
    ARTHRITIS AND RHEUMATISM, 1996, 39 (09): : 1676 - 1676
  • [35] Peroxynitrite modified DNA presents better epitopes for anti-DNA autoantibodies in diabetes type 1 patients
    Tripathi, Prashant
    Moinuddin
    Dixit, Kiran
    Mir, Abdul Rouf
    Habib, Safia
    Alam, Khursheed
    Au, Asif
    CELLULAR IMMUNOLOGY, 2014, 290 (01) : 30 - 38
  • [36] CHARACTERIZATION OF DNA USED TO ASSAY SERA FOR ANTI-DNA ANTIBODIES - DETERMINATION OF SPECIFICITIES OF ANTI-DNA ANTIBODIES IN SLE AND NON-SLE RHEUMATIC DISEASE STATES
    LOCKER, JD
    MEDOF, ME
    BENNETT, RM
    SUKHUPUNYARAKSA, S
    JOURNAL OF IMMUNOLOGY, 1977, 118 (02): : 694 - 701
  • [37] Enhanced binding of circulating SLE autoantibodies to catecholestrogen-copper-modified DNA
    Wahid Ali Khan
    Safia Habib
    Wajid Ali Khan
    Khursheed Alam
    Molecular and Cellular Biochemistry, 2008, 315 : 143 - 150
  • [38] Enhanced binding of circulating SLE autoantibodies to catecholestrogen-copper-modified DNA
    Khan, Wahid Ali
    Habib, Safia
    Khan, Wajid Ali
    Alam, Khursheed
    Moinuddin
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2008, 315 (1-2) : 143 - 150
  • [39] The structural basis for DNA binding by an anti-DNA autoantibody
    Jang, YJ
    Sanford, D
    Chung, HY
    Baek, SY
    Stollar, BD
    MOLECULAR IMMUNOLOGY, 1998, 35 (18) : 1207 - 1217
  • [40] Anti-DNA antibodies in SLEDAI characterised SLE patients
    Stiborova, I
    Kral, V
    Cebecauer, L.
    Blazickova, S.
    ALLERGY, 2010, 65 : 465 - 466
12345