Binding characteristics of SLE anti-DNA autoantibodies to modified DNA analogues

被引：0
|
作者：
Arjumand, S [1 ]
Moinuddin [1 ]
Ali, A [1 ]
机构：
[1] Aligarh Muslim Univ, Fac Med, Dept Biochem, Aligarh, Uttar Pradesh, India
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1997年 / 43卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti-DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity of anti-DNA antibodies for modified conformers was found to be quite high.
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页码：643 / 653
页数：11
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