Detection of genetically modified insect-resistant Bt maize by means of polymerase chain reaction

被引：50
|
作者：
Hupfer, C
Hotzel, H
Sachse, K
Engel, KH
机构：
[1] Tech Univ Munich, Lehrstuhl Allgemeine Lebensmitteltechnol, D-85350 Freising, Germany
[2] Bundesinst Gesundheitlichen Verbraucherschutz & V, D-07743 Jena, Germany
来源：
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY | 1997年 / 205卷 / 06期
关键词：
polymerase chain reaction; genetically modified maize; Bacillus thuringiensis; insect-resistant maize;
D O I：
10.1007/s002170050196
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A method for the detection of genetically modified insect-resistant maize expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The procedure includes: (I) extraction of genomic DNA, (2) amplification of the inserted synthetic gene bq means of the polymerase chain reaction (PCR) method, and (3) characterization and confirmation of the PCR product by Southern hybridization with a digoxigenin-labelled oligo-nucleotide probe and DNA sequencing.
引用
收藏
页码：442 / 445
页数：4
相关论文
共 50 条
  • [31] Detection of the genetic modification in heat-treated products of Bt maize by polymerase chain reaction
    Christine Hupfer
    Helmut Hotzel
    Konrad Sachse
    K.-H. Engel
    Zeitschrift für Lebensmitteluntersuchung und -Forschung A, 1998, 206 : 203 - 207
  • [32] The genetic stability of transgenic Bt and Sck insect-resistant cotton
    Guo Jinying
    Chen Tao
    Zhang Tianzhen
    RECENT ADVANCES IN BIOLOGY, BIOPHYSICS, BIOENGINEERING AND COMPUTATIONAL CHEMISTRY, 2009, : 115 - +
  • [33] Bt protein expression in the transgenic insect-resistant cotton in China
    Guoqing Sun
    Dongling Zhang
    Rui Zhang
    Yuan Wang
    Zhigang Meng
    Tao Zhou
    Chenzhen Liang
    Tao Zhu
    Sandui Guo
    Science Bulletin, 2016, 61 (20) : 1555 - 1557
  • [34] Fate and effects of insect-resistant Bt crops in soil ecosystems
    Icoz, Isik
    Stotzky, Guenther
    SOIL BIOLOGY & BIOCHEMISTRY, 2008, 40 (03): : 559 - 586
  • [35] Bt protein expression in the transgenic insect-resistant cotton in China
    Sun, Guoqing
    Zhang, Dongling
    Zhang, Rui
    Wang, Yuan
    Meng, Zhigang
    Zhou, Tao
    Liang, Chenzhen
    Zhu, Tao
    Guo, Sandui
    SCIENCE BULLETIN, 2016, 61 (20) : 1555 - 1557
  • [36] Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maize
    Cankar, Katarina
    Chauvensy-Ancel, Valerie
    Fortabat, Marie-Noelle
    Gruden, Kristina
    Kobilinsky, Andre
    Zel, Jana
    Bertheau, Yves
    ANALYTICAL BIOCHEMISTRY, 2008, 376 (02) : 189 - 199
  • [37] Detection of genetically modified Bt maize in cooked food products by PCR
    Rizzi, A
    Agosti, F
    Daffonchio, D
    Sorlini, C
    ITALIAN JOURNAL OF FOOD SCIENCE, 2001, 13 (03) : 265 - 273
  • [38] Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean
    Ferrari, Cibele dos Santos
    Valente, Luciana Lehmkuhl
    Brod, Fabio Cristiano Angonesi
    Tagliari, Caroline
    Sant'Anna, Ernani Sebastiao
    Arisi, Ana Carolina Maisonnave
    INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2007, 42 (10): : 1249 - 1255
  • [39] The validation of methods based on polymerase chain reaction for the detection of genetically modified organisms in food
    Anklam, E
    ANALYTICA CHIMICA ACTA, 1999, 393 (1-3) : 177 - 179
  • [40] Multiplex polymerase chain reaction/membrane hybridization assay for detection of genetically modified organisms
    Su, WJ
    Song, SY
    Long, MN
    Liu, GM
    JOURNAL OF BIOTECHNOLOGY, 2003, 105 (03) : 227 - 233
12345