Detection of genetically modified insect-resistant Bt maize by means of polymerase chain reaction

被引：50
|
作者：
Hupfer, C
Hotzel, H
Sachse, K
Engel, KH
机构：
[1] Tech Univ Munich, Lehrstuhl Allgemeine Lebensmitteltechnol, D-85350 Freising, Germany
[2] Bundesinst Gesundheitlichen Verbraucherschutz & V, D-07743 Jena, Germany
来源：
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY | 1997年 / 205卷 / 06期
关键词：
polymerase chain reaction; genetically modified maize; Bacillus thuringiensis; insect-resistant maize;
D O I：
10.1007/s002170050196
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A method for the detection of genetically modified insect-resistant maize expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The procedure includes: (I) extraction of genomic DNA, (2) amplification of the inserted synthetic gene bq means of the polymerase chain reaction (PCR) method, and (3) characterization and confirmation of the PCR product by Southern hybridization with a digoxigenin-labelled oligo-nucleotide probe and DNA sequencing.
引用
收藏
页码：442 / 445
页数：4
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