Persistence of Bluetongue virus serotype 1 virulence in sheep blood refrigerated for 10 years

被引:3
|
作者
Puggioni, Giantonella [1 ]
Pintus, Davide [1 ]
Meloni, Giorgio [1 ]
Scivoli, Rosario [1 ]
Rocchigiani, Angela Maria [1 ]
Manunta, Daniela [1 ]
Savini, Giovanni [2 ]
Oggiano, Annalisa [1 ]
Ligios, Ciriaco [1 ]
机构
[1] Ist Zooprofilatt Sperimentale Sardegna, Via Duca Abruzzi, I-07100 Sassari, Italy
[2] Ist Zooprofilatt Sperimentale Abruzzo & Molise, OIE Reference Lab Bluetongue, I-64100 Campo Boario, Teramo, Italy
关键词
Blood; Bluetongue; Sheep; Storage; DENGUE VIRUS; TEMPERATURE; VIREMIA; STORAGE; ACID;
D O I
10.12834/VetIt.1344.7401.3
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
This paper reports that Bluetongue virus serotype 1 (BTV-1) infected blood collected during the 2006 Sardinia (Italy) epidemic from a ewe with clinical disease and stored at similar to 5 degrees C for 10 years, caused Bluetongue (BT)-like clinical disease and death when inoculated into a susceptible Sarda breed ram. Anatomo-histopathological examination and Real-Time Reverse Transcriptase PCR (Real-Time RT-PCR) confirmed the presence of BTV-1 in several tissues proving that the BTV-1 2006 isolate has maintained its infectivity and virulence.
引用
收藏
页码:349 / 353
页数:5
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