Persistence of Bluetongue virus serotype 1 virulence in sheep blood refrigerated for 10 years

被引：3

作者：

Puggioni, Giantonella ^{[1
]}

Pintus, Davide ^{[1
]}

Meloni, Giorgio ^{[1
]}

Scivoli, Rosario ^{[1
]}

Rocchigiani, Angela Maria ^{[1
]}

Manunta, Daniela ^{[1
]}

Savini, Giovanni ^{[2
]}

Oggiano, Annalisa ^{[1
]}

Ligios, Ciriaco ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Sardegna, Via Duca Abruzzi, I-07100 Sassari, Italy

[2] Ist Zooprofilatt Sperimentale Abruzzo & Molise, OIE Reference Lab Bluetongue, I-64100 Campo Boario, Teramo, Italy

来源：

VETERINARIA ITALIANA | 2018年 / 54卷 / 04期

关键词：

Blood; Bluetongue; Sheep; Storage; DENGUE VIRUS; TEMPERATURE; VIREMIA; STORAGE; ACID;

D O I：

10.12834/VetIt.1344.7401.3

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

This paper reports that Bluetongue virus serotype 1 (BTV-1) infected blood collected during the 2006 Sardinia (Italy) epidemic from a ewe with clinical disease and stored at similar to 5 degrees C for 10 years, caused Bluetongue (BT)-like clinical disease and death when inoculated into a susceptible Sarda breed ram. Anatomo-histopathological examination and Real-Time Reverse Transcriptase PCR (Real-Time RT-PCR) confirmed the presence of BTV-1 in several tissues proving that the BTV-1 2006 isolate has maintained its infectivity and virulence.

引用

页码：349 / 353

页数：5

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